Journal Article

Rat gap junction connexin-30 inhibits proliferation of glioma cell lines

Frédéric Princen, Pierre Robe, Daniel Gros, Thérèse Jarry-Guichard, Jacques Gielen, Marie-Paule Merville and Vincent Bours

in Carcinogenesis

Volume 22, issue 3, pages 507-513
Published in print March 2001 | ISSN: 0143-3334
Published online March 2001 | e-ISSN: 1460-2180 | DOI:
Rat gap junction connexin-30 inhibits proliferation of glioma cell lines

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Connexins, the structural components of gap junctions, control cell growth and differentiation and are believed to belong to a family of tumour suppressor genes. Studies on connexin localization in brain showed that several of these proteins were expressed in distinct compartments of the brain in a cell-type specific manner, indicating that different gap junctions play specific roles in the physiology of the mammalian brain. In this report, we first cloned rat connexin-30 cDNA from brain and showed that it was expressed in long-term primary culture of rat astrocytes. In order to examine the potential role of connexin-30 in tumour cell proliferation, we transfected the connexin-30 cDNA into two rat glioma cell lines (9L and C6) which have lost its expression. Transfected clones adequately expressed membrane-bound connexin-30 protein. Connexin-30-expressing clones showed slower growth, lower DNA synthesis and reduced proliferation in soft agar as compared with the parental and control cells. We concluded that connexin-30 may also probably be considered as a tumour suppressor in rat gliomas.

Keywords: FITC, fluorescein isothiocyanate; GJIC, gap junctional intercellular communication; MTN, multiple tissue northern; TBS-T, Tris-buffered saline–Tween 20; TRITC, tetramethylrhodamine isothiocyanate.

Journal Article.  5450 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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