Journal Article

Antitumorigenic activity of the prebiotic inulin enriched with oligofructose in combination with the probiotics <i>Lactobacillus rhamnosus</i> and <i>Bifidobacterium lactis</i> on azoxymethane-induced colon carcinogenesis in rats

Angelo Pietro Femia, Cristina Luceri, Piero Dolara, Augusto Giannini, Annibale Biggeri, Maddalena Salvadori, Yvonne Clune, Kevin J. Collins, Milena Paglierani and Giovanna Caderni

in Carcinogenesis

Volume 23, issue 11, pages 1953-1960
Published in print November 2002 | ISSN: 0143-3334
Published online November 2002 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/23.11.1953
Antitumorigenic activity of the prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobacterium lactis on azoxymethane-induced colon carcinogenesis in rats

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Prebiotics such as fructans, and probiotics such as Lactobacilli or Bifidobacteria, or a combination of prebiotics and probiotics (synbiotics) are thought to be protective against colon cancer. Therefore, we studied whether the prebiotic inulin enriched with oligofructose (Raftilose-Synergy1®, briefly, Synergy1, 10% of the diet), probiotics [Bifidobacterium lactis (Bb12) and Lactobacillus rhamnosus (LGG), each at 5×108 c.f.u./g diet] or synbiotics (a combination of the two) protect rats against azoxymethane (AOM)-induced colon cancer. Male F344 rats were divided into: Controls; PRE, which were fed a diet containing Synergy1; PRO, fed a diet containing LGG and Bb12; PREPRO, fed a diet containing Synergy1, LGG and BB12. Ten days after beginning the diets, rats were treated with AOM (15 mg/kg s.c. two times); dietary treatments were continued for the entire experiment. Thirty-one weeks after AOM, rats treated with Synergy1 (PRE and PREPRO groups) had a significantly lower (P < 0.001) number of tumours (adenomas and cancers) than rats without Synergy1 (colorectal tumours/rat were 1.9 ± 1.7, 1.1 ± 1.1, 2.2 ± 1.4 and 0.9 ± 1.2 in Controls, PRE, PRO and PREPRO groups, respectively, means ± SD). A slight, not significant effect of probiotics in reducing malignant tumours was also observed (P = 0.079). Caecal short-chain fatty acids (SCFA) were higher (P < 0.001) in the groups treated with Synergy1. Apoptosis was increased in the normal mucosa of the PRO group, while no variation was observed in the tumours. Colonic proliferation was lower in the PRE group as compared with Controls. Glutathione S-transferase placental enzyme pi type expression, and to a lesser extent, inducible NO synthase were depressed in the tumours from rats in the PRE and PREPRO groups. Cycloxygenase-2 expression was increased in the tumours of control rats but not in those from PRE, PRO or PREPRO rats. In conclusion, prebiotic administration in the diet decreases AOM-induced carcinogenesis in rats.

Keywords: ACF, aberrant crypt foci; AI, apoptotic index; AOM, azoxymethane; Bb12, Bifidobacterium lactisBb12; COX-2, cyclooxygenase-2; GST-P, GST placental enzyme pi type; HF, high fat; LGG, Lactobacillus rhamnosus; PCNA, proliferating cell nuclear antigen; SCFA, short-chain fatty acids.

Journal Article.  8141 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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