Journal Article

Expression of cyclin D1/2 in the lungs of strain A/J mice fed chemopreventive agents

Hanspeter Witschi, Imelda Espiritu, Marie Suffia and Kent E. Pinkerton

in Carcinogenesis

Volume 23, issue 2, pages 289-294
Published in print February 2002 | ISSN: 0143-3334
Published online February 2002 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/23.2.289
Expression of cyclin D1/2 in the lungs of strain A/J mice fed chemopreventive agents

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Male strain A mice were fed a diet containing chemopreventive agents. After 1 and 3 weeks on the diets, lung nuclear fractions were examined for expression of cyclin D1/2 with western blot analysis. In animals fed a diet containing a mixture of myoinositol and dexamethasone, a treatment found previously to be effective in preventing the development of tobacco smoke-induced lung tumors in A/J mice, cyclin D1/2 expression was reduced to 30–40% of control levels. A similar decrease in cyclin D1/2 expression was found when animals were fed either myoinositol or dexamethasone alone. Paradoxically, tobacco smoke by itself had a similar effect on cyclin D1/2 expression. On the other hand, several agents that had been previously found not to be effective against tobacco smoke carcinogenesis [phenethyl isothiocyanate, 1,4-phenylenebis(methylene)selenoisocyanate, N-acetylcysteine, acetylsalicylic acid, D-limonene and beta carotene] did not decrease cyclin D1/2 expression after 1 or 3 weeks of feeding. It was concluded that expression of cyclin D1/2 might be a potentially useful marker in the identification of chemopreventive agents for tobacco smoke and could be of some help in the evaluation of their effects.

Keywords: ASA, acetylsalicylic acid (aspirin); CDK, cyclin-dependent kinases; NAC, N-acetylcysteine; PEITC, phenethyl isothiocyanate; pXSC, 1,4-phenylenebis(methylene)selenoisocyanate; TSP, total suspended particulates.

Journal Article.  5077 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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