Journal Article

Allyl isothiocyanate, a constituent of cruciferous vegetables, inhibits proliferation of human prostate cancer cells by causing G<sub>2</sub>/M arrest and inducing apoptosis

Dong Xiao, Sanjay K. Srivastava, Karen L. Lew, Yan Zeng, Pamela Hershberger, Candace S. Johnson, Donald L. Trump and Shivendra V. Singh

in Carcinogenesis

Volume 24, issue 5, pages 891-897
Published in print May 2003 | ISSN: 0143-3334
Published online May 2003 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgg023
Allyl isothiocyanate, a constituent of cruciferous vegetables, inhibits proliferation of human prostate cancer cells by causing G2/M arrest and inducing apoptosis

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics

GO

Show Summary Details

Preview

Dietary isothiocyanates (ITCs) are highly effective in affording protection against chemically induced cancers in laboratory animals. In the present study, we demonstrate that allyl isothiocyanate (AITC), a constituent of cruciferous vegetables, significantly inhibits proliferation of cultured PC-3 (androgen-independent) and LNCaP (androgen-dependent) human prostate cancer cells in a dose-dependent manner with an IC50 of ∼15–17 µM. On the other hand, survival of a normal prostate epithelial cell line (PrEC) was minimally affected by AITC even at concentrations that were highly cytotoxic to the prostate cancer cells. Reduced proliferation of PC-3 as well as LNCaP cells in the presence of AITC correlated with accumulation of cells in G2/M phase and induction of apoptosis. In contrast, AITC treatment failed to induce apoptosis or cause G2/M phase arrest in PrEC cells. A 24 h treatment of PC-3 and LNCaP cells with 20 μM AITC caused a significant decrease in the levels of proteins that regulate G2/M progression, including Cdk1 (32–50% reduction), Cdc25B (44–48% reduction) and Cdc25C (>90% reduction). A significant reduction in the expression of cyclin B1 protein (∼45%) was observed only in LNCaP cells. A 24 h exposure of PC-3 and LNCaP cells to an apoptosis-inducing concentration of AITC (20 μM) resulted in a significant decrease (31–68%) in the levels of anti-apoptotic protein Bcl-2 in both cell lines, and ∼58% reduction in Bcl-XL protein expression in LNCaP cells. In conclusion, it seems reasonable to hypothesize that AITC, and possibly other ITCs, may find use in the treatment of human prostate cancers.

Keywords: AITC, allyl isothiocyanate; Cdk1, cyclin-dependent kinase 1; ITCs, isothiocyanates; PEITC, phenethyl isothiocyanate; PrEC, prostate epithelial cell line.

Journal Article.  4780 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.