Journal Article

The alleles of the DNA repair gene <i>O</i><sup>6</sup>-alkylguanine-DNA alkyltransferase are expressed at different levels in normal human lung tissue

J. Heighway, G.P. Margison and M.F. Santibáñez-Koref

in Carcinogenesis

Volume 24, issue 10, pages 1691-1694
Published in print October 2003 | ISSN: 0143-3334
Published online October 2003 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgg124
The alleles of the DNA repair gene O6-alkylguanine-DNA alkyltransferase are expressed at different levels in normal human lung tissue

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O6-Alkylguanine-DNA alkyltransferase (MGMT) confers resistance to many of the mutagenic and toxic effects of certain classes of alkylating agents by repairing the DNA lesions responsible. The levels of expression of this protein are of interest in relation to the prevention and treatment of cancer in man. They vary widely between individuals, and the basis of this variation is not understood. RT–PCR–RFLP analysis of mRNA from normal human lung tissue reveals that the two MGMT alleles are frequently expressed at different levels, indicating that there is a genetic component to inter-individual variation of MGMT levels and that at least some of this variation maps close to or within the MGMT locus.

Keywords: AEI, allelic expression imbalance; MGMT, O6-alkylgua- nine-DNA alkyltransferase; NNK, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone; NNN, N-nitrosonornicotine

Journal Article.  2608 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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