Journal Article

Differential gene expression in rat colon by dietary heme and calcium

C. van der Meer-van Kraaij, E. Kramer, D. Jonker-Termont, M.B. Katan, R. van der Meer and J. Keijer

in Carcinogenesis

Volume 26, issue 1, pages 73-79
Published in print January 2005 | ISSN: 0143-3334
Published online January 2005 | e-ISSN: 1460-2180 | DOI:
Differential gene expression in rat colon by dietary heme and calcium

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Dietary heme and calcium are alleged modulators of colon cancer risk. Little is known about the molecular and cellular changes in the colon epithelium that are induced by consumption of these unabsorbed nutrients. In this nutrigenomics study, we fed rats high- and low-calcium diets with or without heme. In agreement with previous studies, we found that dietary heme increased the cytotoxicity of fecal water in the colon and elevated epithelial proliferation, a risk factor in colon carcinogenesis. Calcium reduced cytotoxicity and inhibits heme-induced effects. Among 365 colon-expressed genes, we could identify 10 diet-modulated genes that show >2-fold altered expression, of which several are related to colon cell turnover and disease. Mucosal pentraxin (Mptx) was the strongest differentially expressed gene, ∼10-fold down-regulated by dietary heme and 3-fold up-regulated by calcium. cDNA microarray and quantitative PCR analysis show that calcium significantly inhibits the effects of heme, which correlates with the physiological effects. Our results indicate that Mptx expression is related to colonic cell turnover, and that Mptx might be a marker for diet-modulated mucosal integrity. We also show that Mptx expression is restricted to the intestine, and occurs predominantly in the colon.

Keywords: Aldoa, aldolase A; CA1, carbonic anhydrase I gene; CRP, C-reactive protein; Krt21, cytokeratin 21; Mptx, mucosal pentraxin; Fxyd4, FXYD ion transport regulator 4; PCA, principal component analysis; SAP, serum amyloid P component

Journal Article.  6103 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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