Journal Article

Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis

Mei-Hui Tai, Chia-Cheng Chang, L.Karl Olson and James E. Trosko

in Carcinogenesis

Volume 26, issue 2, pages 495-502
Published in print February 2005 | ISSN: 0143-3334
Published online February 2005 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgh321
Oct4 expression in adult human stem cells: evidence in support of the stem cell theory of carcinogenesis

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The Oct3/4 gene, a POU family transcription factor, has been noted as being specifically expressed in embryonic stem cells and in tumor cells but not in cells of differentiated tissues. With the ability to isolate adult human stem cells it became possible to test for the expression of Oct3/4 gene in adult stem cells and to test the stem cell theory of carcinogenesis. Using antibodies and PCR primers we tested human breast, liver, pancreas, kidney, mesenchyme and gastric stem cells, the cancer cell lines HeLa and MCF-7 and human, dog and rat tumors for Oct4 expression. The results indicate that adult human stem cells, immortalized non-tumorigenic cells and tumor cells and cell lines, but not differentiated cells, express Oct4. Oct4 is expressed in a few cells found in the basal layer of human skin epidermis. The data demonstrate that adult stem cells maintain expression of Oct4, consistent with the stem cell hypothesis of carcinogenesis.

Keywords: BPE, bovine pituitary extract; cpdl, cumulative population doublings; DAPI, 4′,6′-diamidino-2-phenylindole dihydrochloride; EGF, epidermal growth factor; ES, embryonic stem; GJIC, gap junctional intercellular communication; HBEC, human breast epithelial cell; HPCs, human pancreatic stem cells; PBS, phosphate buffered-saline

Journal Article.  6255 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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