Journal Article

Penta-<i>O</i>-galloyl-beta-<span class="smallCaps">d</span>-glucose suppresses tumor growth via inhibition of angiogenesis and stimulation of apoptosis: roles of cyclooxygenase-2 and mitogen-activated protein kinase pathways

Jeong-Eun Huh, Eun-Ok Lee, Min-Seok Kim, Kyung-Sun Kang, Cheol-Ho Kim, Bae-Cheon Cha, Young-Joon Surh and Sung-Hoon Kim

in Carcinogenesis

Volume 26, issue 8, pages 1436-1445
Published in print August 2005 | ISSN: 0143-3334
Published online April 2005 | e-ISSN: 1460-2180 | DOI:
Penta-O-galloyl-beta-d-glucose suppresses tumor growth via inhibition of angiogenesis and stimulation of apoptosis: roles of cyclooxygenase-2 and mitogen-activated protein kinase pathways

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Recent studies have revealed that 1,2,3,4,6-penta-O-galloyl-beta-d-glucose (PGG) has anti-tumorigenic activity in vitro. In the present work, we evaluated the in vitro and in vivo antiangiogenic and antitumor activities of PGG and examined its molecular mechanisms. PGG significantly inhibited the proliferation and tube formation in basic fibroblast growth factor (bFGF)-treated human umbilical vein endothelial cells (HUVECs) at non-cytotoxic concentrations. PGG effectively disrupted the bFGF-induced neo-vascularization in chick chorioallantoic membrane (CAM) and in Matrigel plugs in the mice. When mice were intraperitoneally injected, PGG also significantly inhibited tumor angiogenesis induced by Lewis lung carcinoma (LLC) and the growth of LLC by 57 and 91% of control tumor weight at 4 and 20 mg/kg, respectively. Immunohistochemical analysis revealed decreased microvessel density, decreased expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF), reduced tumor cell proliferation and increased tumor cell apoptosis. Similarly, PGG significantly attenuated the expression of COX-2 and VEGF and reduced the secretion of VEGF and prostaglandin E2 in bFGF-treated HUVECs. Furthermore, the COX-2 inhibitor NS398 significantly inhibited tube formation and neo-vascularization in CAM, supporting the role of COX-2 in PGG inhibition of angiogenesis. PGG diminished the phosphorylation of extracellular signal regulated kinase 1/2, Jun NH2-terminal kinase and activated phospho-p38 mitogen-activated protein kinase (MAPK) in a dose-dependent manner in bFGF-treated HUVECs. In addition, p38 inhibitor SB203580 abolished the downregulation of COX-2, VEGF and the antiproliferative activity by PGG. Taken together, our data demonstrate that PGG exerts antitumor activity primarily via inhibition of angiogenesis through COX-2 and MAPK- dependent pathways.

Keywords: bFGF, basic fibroblast growth factor; CAM, Chick chorioallantoic membrane; COX-2, cyclooxygenase-2; ERK, extracellular signal regulated kinase 1/2; HUVECs, human umbilical vein endothelial cells; JNK, Jun NH2-terminal kinase; LLC, Lewis lung carcinoma; MAPK, mitogen activated protein kinase; PCNA, proliferating cell nuclear antigen; PGE2, prostaglandin E2; PGG, 1,2,3,4,6-penta-O-galloyl-beta-d-glucose; VEGF, vascular endothelial growth factor

Journal Article.  5179 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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