Journal Article

Effect of inflammation on cyclooxygenase (COX)-2 expression in benign and malignant oesophageal cells

Salem I. Abdalla, Ian R. Sanderson and Rebecca C. Fitzgerald

in Carcinogenesis

Volume 26, issue 9, pages 1627-1633
Published in print September 2005 | ISSN: 0143-3334
Published online May 2005 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgi114
Effect of inflammation on cyclooxygenase (COX)-2 expression in benign and malignant oesophageal cells

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics

GO

Show Summary Details

Preview

Chronic inflammation has been linked to carcinogenesis in various tissue sites. Barrett's oesophageal epithelium (BE) is a premalignant condition in which cyclooxygenase-2 (COX-2) levels are increased. However, it is not clear whether the primary stimulus for the high COX-2 levels is related to inflammation or malignancy. The effect of exogenous cytokines (IL-1β, IL-10 and IL-13) on COX-2 expression was assessed by western blotting in three BE cancer cell lines (SEG-1, BIC-1 and OE33) and a squamous cancer cell line (OE21). Primary tissue was assessed from 17 patients with long BE segments, 13 oesophagitis, 30 oesophageal adenocarcinoma (EAC), and 40 normal oesophageal (NE) and duodenal (DU) controls. COX-2 protein expression was determined by western blotting and its tissue localization was examined using immunohistochemistry. COX-2 protein and the neutrophil marker myeloperoxidase (MPO) were quantified along BE segments. The leukocyte marker CD45 was used to identify any correlation between COX-2 expression and leukocyte cell distribution in EAC. IL-1β induced COX-2 expression in SEG-1 cells (P < 0.05), whereas IL-10 and IL-13 had no effect. COX-2 protein levels were found to be increased in distal BE > proximal BE > oesophagitis > NE (P < 0.001). COX-2 expression in EAC was heterogeneous and the overall levels were not significantly increased. The increased COX-2 expression in distal BE was not associated with inflammation (MPO expression). In addition, there was no correlation between COX-2 and CD45 in AC. COX-2 protein expression in the oesophagus appears to be independent of the degree of inflammation.

Keywords: BE, Barrett's oesophageal epithelium; COX-2, cyclooxygenase-2 enzyme; DU, duodenum; EAC, oesophageal adenocarcinoma; EGF, epidermal growth factor; GOJ, gastro-oesophageal junction; IL, interleukin; MPO, myeloperoxidase; NE, normal squamous oesophageal epithelium; NFκB, nuclear factor κ-B; NSAIDs, non-steroidal anti-inflammatory drugs; PPIs, proton pump inhibitors; TGF-β, transforming growth factor-beta; TNF-α, tumour necrosis factor-alpha.

Journal Article.  4875 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.