Journal Article

An animal model for the rapid induction of tongue neoplasms in human c-Ha-<i>ras</i> proto-oncogene transgenic rats by 4-nitroquinoline 1-oxide: its potential use for preclinical chemoprevention studies

Rikako Suzuki, Hiroyuki Kohno, Masumi Suzui, Naoki Yoshimi, Hiroyuki Tsuda, Keiji Wakabayashi and Takuji Tanaka

in Carcinogenesis

Volume 27, issue 3, pages 619-630
Published in print March 2006 | ISSN: 0143-3334
Published online October 2005 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgi241
An animal model for the rapid induction of tongue neoplasms in human c-Ha-ras proto-oncogene transgenic rats by 4-nitroquinoline 1-oxide: its potential use for preclinical chemoprevention studies

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Oral squamous cell carcinoma is one of the most common human neoplasms, and prevention of this malignancy requires a better understanding of its carcinogenesis process. To this end, we tried to establish an animal model using the human c-Ha-ras proto-oncogene-carrying transgenic (Tg) rats and the carcinogen 4-nitroquinoline 1-oxide (4-NQO). 4-NQO (20 p.p.m.) was administered to Tg and non-Tg rats for 8 weeks in their drinking water, and then the occurrence of tongue carcinogenesis was compared during the experimental period of 22 weeks. In addition, we determined the DNA ploidy in tongue lesions and examined the immunohistochemical expression of five biomarkers such as cyclin D1, glutathione S-transferase placental form, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) and β-catenin. Next, the cancer chemopreventive effects of nimesulide, pioglitazone and a synthetic geranylated derivative, which have been reported to be inhibitors of tongue carcinogenesis, were examined in Tg rats treated with 4-NQO. Either during or after treatment with 4-NQO in the drinking water, tongue dysplasia and tumors were observed on the tongues of both Tg and non-Tg rats, with a greater incidence and multiplicity in Tg rats. Histopathologically, squamous cell dysplasia, papilloma and carcinoma with or without invasion were present in the tongue. Immunohistochemistry revealed that expression levels against five biomarkers increase with disease progression, and the changes correlated with those of the DNA ploidy pattern. Interestingly, a strong expression of COX-2, iNOS and β-catenin was observed on the invasive front of squamous cell carcinomas. A subsequent chemoprevention study using Tg rats showed that the chemicals tested suppressed the occurrence of tongue carcinomas when they were administered after 4-NQO-exposure. These results may thus indicate that our 4-NQO-induced Tg rat tongue carcinogenesis model simulates many aspects of human oral carcinogenesis and it can be applied for an analysis of oral cancer development while also helping to identify potentially effective cancer chemopreventive agents against oral cancer.

Keywords: CDK, cyclin-dependent kinase; COX, cyclooxygenase; DMBA, 7,12-dimethylbenz(a)anthracene; EGMP, ethyl 3-(4′-geranyloxy-3′-methoxyphenyl)-2-propenoate; GST-P, glutathione S-transferase placental form; H&E, hematoxylin and eosin; iNOS, inducible nitric synthase; NO, Nitric oxide; 4-NQO, 4-nitroquinoline 1-oxide; PPAR, peroxisome proliferator-activated receptor; SCC, squamous cell carcinoma; Tg rats, Human c-Ha-ras proto-oncogene carrying transgenic rats

Journal Article.  7684 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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