Journal Article

Cholinesterase activity of human lung tumours varies according to their histological classification

Pedro Martínez-Moreno, Susana Nieto-Cerón, Juan Torres-Lanzas, Francisco Ruiz-Espejo, Isabel Tovar-Zapata, Pedro Martínez-Hernández, José N. Rodríguez-López, Cecilio J. Vidal and Juan Cabezas-Herrera

in Carcinogenesis

Volume 27, issue 3, pages 429-436
Published in print March 2006 | ISSN: 0143-3334
Published online November 2005 | e-ISSN: 1460-2180 | DOI:
Cholinesterase activity of human lung tumours varies according to their histological classification

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics


Show Summary Details


The probable involvement of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in cancer and the relevance of cholinergic responses for lung cancer growth prompted us to study whether cholinesterase activity of human lung is altered by malignancy. Surgical pieces of non-small lung carcinomas (NSLC) and their adjacent non-cancerous tissues (ANCT) were analysed for AChE and BChE activities. AChE activity in adenocarcinoma (AC) was 7.80 ± 5.59 nmol of substrate hydrolysed per min and per mg of protein (mU/mg), the same as in their ANCT (8.83 ± 4.72 mU/mg; P = 0.823); in large cell carcinoma (LCC), 7.52 ± 3.32 mU/mg, ∼50% less than in their ANCT (15.39 ± 5.66 mU/mg; P = 0.043); and in squamous cell carcinoma (SCC), 1.39 ± 0.58 mU/mg, 80% less than in ANCT (6.08 ± 2.88 mU/mg; P = 0.003). BChE activity was 5.85 ± 3.20 mU/mg in AC and 9.56 ± 3.38 mU/mg in ANCT (P = 0.022); 2.94 ± 2.01 mU/mg in LCC and 6.50 ± 6.63 mU/mg in ANCT (P = 0.068); and 4.49 ± 2.30 mU/mg in SCC and ANCT 6.56 ± 4.09 mU/mg (P = 0.026). Abundant AChE dimers and fewer monomers were identified in lung and, although their distribution was unaffected by cancer, the binding with concanavalin A revealed changes in AChE glycosylation between SCC and their ANCT. The fall in BChE activity affected all molecules, with a strong decrease of the amphiphilic tetramers. Western blotting revealed protein bands with the expected mass of the principal AChE subunits, and the deeper intensity of the protein signal in SCC than in healthy lung, in lanes loaded with the same units of AChE activity, supported an augment in the amount of AChE protein/unit of AChE activity in SCC. The increased availability of acetylcholine in neoplastic lung, resulting from the fall of cholinesterase activity, may enhance cholinergic signalling and contribute to tumour progression.

Keywords: AC, adenocarcinoma; ACh, acetylcholine; AChE, acetylcholinesterase; ANCT, adjacent non-cancerous tissue; BChE, butyrylcholinesterase; ChE, cholinesterase; ChAT, choline acetyltransferase; GPI, glycosylphosphatidylinositol; LCC, large cell carcinomas; NSCLC, non-small cell lung carcinomas; SCLC, small cell lung carcinomas; SCC, squamous cell carcinomas

Journal Article.  7106 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.