Journal Article

Effect of short-term fasting on urinary excretion of primary lipid peroxidation products and on markers of oxidative DNA damage in healthy women

Kyoung-Ho Lee, Helmut Bartsch, Jagadeesan Nair, Dong-Ho Yoo, Yun-Chul Hong, Soo-Hun Cho and Daehee Kang

in Carcinogenesis

Volume 27, issue 7, pages 1398-1403
Published in print July 2006 | ISSN: 0143-3334
Published online January 2006 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgi337
Effect of short-term fasting on urinary excretion of primary lipid peroxidation products and on markers of oxidative DNA damage in healthy women

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The goal of this study was to determine whether short-term fasting changes in urinary biomarkers related to oxidative stress: malondialdehyde (MDA), 8-isoprostaglandin F (8-isoPGF), 8-hydroxydeoxy-guanosine (8-OHdG) and 1,N6-ethenodeoxyadenosine (εdA) among female volunteers participating in the short-term fasting program in South Korea. The study subjects were 52 healthy women (mean age 28, range 15–48 years old) who provided urine samples both before and after the fasting program (average 7.2, range: 3–11 days). Urinary MDA was measured by HPLC-UV and εdA levels were measured by immuno-affinity purification followed by HPLC-fluorescence detection. Urinary 8-OHdG and 8-isoPGF concentrations were determined by ELISA. Plasma leptin levels were also measured by radioimmunoassay. Information on demographic characteristics, personal habits (smoking and alcohol consumption) and previous medical history were collected by a self-administered questionnaire. Percent loss of body weight (average 6.3%, 4.28 ± 0.25 kg) was significantly correlated with fasting duration (r = 0.70, n = 52, P < 0.01). The plasma leptin levels after fasting (5.89 ± 1.10 ng/ml) were significantly lower than before fasting (6.91 ± 1.13 ng/ml) (n = 27, P = 0.05). Urinary MDA levels after fasting (0.18 ± 1.10 mg/g creatinine) were significantly lower than before fasting (0.37 ± 1.11) (n = 51, P < 0.01). Urinary 8-isoPGF also were significantly reduced after fasting (n = 47, P < 0.01). However, there was no significant difference in 8-OHdG or εdA. There was a statistically significant correlation between % change of urinary MDA level with % change of 8-isoPGF level (partial correlation coefficient r = 0.57, n = 46, P = 0.01). The correlations between % change of 8-OHdG and plasma leptin was also significant (partial correlation coefficient r = 0.51, n = 27, P = 0.02). Our results demonstrate that the short-term fasting reduces lipid peroxidation products but does not affect oxidative stress-induced DNA damage.

Keywords: 8-isoPGF, 8-isoprostaglandin-F2α; 8-OHdG, 8-oxo-7,8-dihydro-2′-deoxyguanosine; ELISA, enzyme-linked immunosorbent assay; LPO, lipid peroxidation; MDA, malondialdehyde; TBA, 2-thiobarbituric acid; εdA, 1,N6-ethenodeoxyadenosine

Journal Article.  3541 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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