Journal Article

Fractionation of grape seed extract and identification of gallic acid as one of the major active constituents causing growth inhibition and apoptotic death of DU145 human prostate carcinoma cells

Ravikanth Veluri, Rana P. Singh, Zhengjie Liu, John A. Thompson, Rajesh Agarwal and Chapla Agarwal

in Carcinogenesis

Volume 27, issue 7, pages 1445-1453
Published in print July 2006 | ISSN: 0143-3334
Published online February 2006 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgi347
Fractionation of grape seed extract and identification of gallic acid as one of the major active constituents causing growth inhibition and apoptotic death of DU145 human prostate carcinoma cells

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The anti-cancer efficacy of grape seed extract (GSE) against prostate cancer (PCA) via its anti-proliferative, pro-apoptotic and anti-angiogenic activities in both cell culture and animal models have recently been described by us. GSE is a complex mixture containing gallic acid (GA), catechin (C), epicatechin (EC) and several oligomers (procyanidins) of C and/or EC, some of which are esterified to GA. To determine which components are most active against PCA, an ethyl acetate extract of GSE was separated by reverse-phase high-performance liquid chromatography (HPLC) into three fractions. Fraction 1 was far more effective than others in causing growth inhibition and apoptotic death of human PCA DU145 cells. Of the components in this fraction, GA showed a very strong dose- and time-dependent growth inhibition and apoptotic death of DU145 cells, but C and procyanidins B1 (EC–C dimer), B2 (EC–EC dimer) and B3 (C–C dimer) were nearly ineffective. Mechanistic studies demonstrated a strong caspase-9, caspase-3 and poly (ADP-ribose) polymerase (PARP) cleavages by GA in DU145 cells. Procyanidin oligomers eluting in HPLC Fractions 2 and 3 were obtained in larger quantities by separating GSE into eight fractions (I–VIII) on a gel filtration column. All fractions were analyzed by HPLC-UV and negative-ion electrospray mass spectrometry. Fractions I–III contained the active compound GA and inactive components C, EC, B1 and B2. Fraction IV contained other dimers and a dimer–GA ester and was also less active than GSE in DU145 cells. Fractions V–VIII, however, caused significant growth inhibition and apoptosis with the highest activity present in the later fractions that contained procyanidin trimers and GA esters of dimers and trimers. Together, these observations identify GA as one of the major active constituents in GSE. Several procyanidins, however, and especially the gallate esters of dimers and trimers also may be efficacious against PCA and merit further investigation.

Keywords: DMSO, dimethyl sulfoxide; FACS, florescence-activated cell sorting; GA, gallic acid; GSE, grape seed extract; HPLC, high-performance liquid chromatography; LC/MS, liquid chromatography/mass spectrometry; PARP, poly (ADP-ribose) polymerase; PCA, prostate cancer; PI, propidium iodide

Journal Article.  6837 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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