Journal Article

Interferon-β treatment of cervical keratinocytes naturally infected with human papillomavirus 16 episomes promotes rapid reduction in episome numbers and emergence of latent integrants

M.Trent Herdman, Mark R. Pett, Ian Roberts, William O.F. Alazawi, Andrew E. Teschendorff, Xiao-Yin Zhang, Margaret A. Stanley and Nicholas Coleman

in Carcinogenesis

Volume 27, issue 11, pages 2341-2353
ISSN: 0143-3334
Published online September 2006 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgl172
Interferon-β treatment of cervical keratinocytes naturally infected with human papillomavirus 16 episomes promotes rapid reduction in episome numbers and emergence of latent integrants

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Following integration of human papillomavirus (HPV) into the host genome, overexpression of the viral oncogenes E6 and E7 requires loss of the transcriptional repressor functions of E2. A key step in HPV-related carcinogenesis is therefore clearance of residual viral episomes, which encode E2. As spontaneous loss of HPV-16 episomes in vitro is associated with increased expression of antiviral genes inducible by type I interferon (IFN), we used the W12 model to examine the effects of exogenous IFN-β on cervical keratinocytes containing HPV-16 episomes as a result of ‘natural’ infection in vivo. In contrast to studies of cells transfected with HPV-31 or bovine papillomavirus, IFN-β caused rapid reduction in numbers of HPV-16 episomes. This was associated with the emergence of cells bearing previously latent integrants, in which there was increased expression of E6 and E7. Our data indicate that integrated HPV-16 can exist in a minority of cells in a mixed population without exerting a selective advantage until episome numbers are reduced. The kinetics of cell death and changes in viral transcription and translation that we observed support a model where integrants are initially present in cells also containing episomes, with generalized episome clearance by IFN-β resulting in integrant de-repression. We conclude that IFN-β can hasten the transition from episomal to integrated HPV-16 in naturally infected cervical keratinocytes. Greater emphasis should be placed on episome loss in models of HPV-related carcinogenesis. We provide the strongest evidence to date that treating HPV-16 lesions by inducing an IFN response may cause clinical progression.

Journal Article.  9248 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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