Journal Article

Induction of inducible nitric oxide synthase: a protective mechanism in colitis-induced adenocarcinoma

Rui Zhang, Adrienne Ma, Stefan J. Urbanski and Donna-Marie McCafferty

in Carcinogenesis

Volume 28, issue 5, pages 1122-1130
Published in print May 2007 | ISSN: 0143-3334
Published online November 2006 | e-ISSN: 1460-2180 | DOI:
Induction of inducible nitric oxide synthase: a protective mechanism in colitis-induced adenocarcinoma

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A close association between inflammatory bowel disease (IBD) and increased risk of developing adenocarcinoma exists. Moreover, chronic induction of high levels of nitric oxide (NO) produced from inducible nitric oxide synthase (iNOS) is a consistent observation in IBD. In this study we made interleukin-10/inducible nitric oxide synthase double-deficient (IL-10−/−/iNOS−/−) mice and studied the development of adenocarcinoma. Mice >6 months of age were compared with healthy wild-type (WT) controls. Inflammation was assessed using macroscopic/histological scores and myeloperoxidase activity as an indication of granulocyte infiltration. Mucosal polyps were scored macroscopically and hyperproliferation was quantified by 5-bromo-2-deoxyuridine immunohistochemistry. Dysplastic changes were assessed histologically based on cytologic and architectured atypia as well as the presence of submucosal invasion. The p53 and β-catenin messenger RNA mRNA and protein expression were assessed using real-time polymerase chain reaction and immunohistochemistry, respectively.

Inflammatory indices were significantly elevated in interleukin-10-deficient (IL-10−/−) over WT and not significantly different from IL-10−/−/iNOS−/− mice. The incidence of mucosal polyps was similar between IL-10−/− (79%) and IL-10−/−/iNOS−/− (83%) mice; however, significantly higher numbers of polyps were observed in the absence of iNOS (P < 0.05). Hyperproliferation was noted in both groups. Signs of dysplasia and submucosal invasion were significantly higher in IL-10−/−/iNOS−/− compared with IL-10−/− mice (P < 0.05). No significant increase in p53 and β-catenin mRNA levels was observed in IL-10−/− over WT mice; however, a 2-fold (P = 0.06) and 3-fold (P < 0.05) increase, respectively, was noted in IL-10−/−/iNOS−/− mice. Our data suggest exposure to chronic NO limits abnormal p53 and β-catenin expression and reduces incidence of adenocarcinoma in IL-10−/− mice.

Journal Article.  6016 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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