Journal Article

Natural chlorophyll inhibits aflatoxin B<sub>1</sub>-induced multi-organ carcinogenesis in the rat

Michael T. Simonich, Patricia A. Egner, Bill D. Roebuck, Gayle A. Orner, Carole Jubert, Cliff Pereira, John D. Groopman, Thomas W. Kensler, Roderick H. Dashwood, David E. Williams and George S. Bailey

in Carcinogenesis

Volume 28, issue 6, pages 1294-1302
Published in print June 2007 | ISSN: 0143-3334
Published online February 2007 | e-ISSN: 1460-2180 | DOI:
Natural chlorophyll inhibits aflatoxin B1-induced multi-organ carcinogenesis in the rat

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics


Show Summary Details


Chemoprevention by chlorophyll (Chl) was investigated in a rat multi-organ carcinogenesis model. Twenty-one male F344 rats in three gavage groups (N = 7 rats each) received five daily doses of 250 μg/kg [3H]-aflatoxin B1 ([3H]-AFB1) alone, or with 250 mg/kg chlorophyllin (CHL), or an equimolar amount (300 mg/kg) of Chl. CHL and Chl reduced hepatic DNA adduction by 42% (P = 0.031) and 55% (P = 0.008), respectively, AFB1–albumin adducts by 65% (P < 0.001) and 71% (P < 0.001), respectively, and the major AFB–N7-guanine urinary adduct by 90% (P = 0.0047) and 92% (P = 0.0029), respectively. To explore mechanisms, fluorescence quenching experiments established formation of a non-covalent complex in vitro between AFB1 and Chl (Kd = 1.22 ± 0.05 μM, stoichiometry = 1Chl:1AFB1) as well as CHL (Kd = 3.05 ± 0.04 μM; stoichiometry = 1CHL:1AFB1). The feces of CHL and Chl co-gavaged rats contained 137% (P = 0.0003) and 412% (P = 0.0048) more AFB1 equivalents, respectively, than control feces, indicating CHL and Chl inhibited AFB1 uptake. However, CHL or Chl treatment in vivo did not induce hepatic quinone reductase (NAD(P)H:quinone oxidoreductase) or glutathione S-transferase (GST) above control levels. These results are consistent with a mechanism involving complex-mediated reduction of carcinogen uptake, and do not support a role for phase II enzyme induction in vivo under these conditions. In a second study, 30 rats in three experimental groups were dosed as in study 1, but for 10 days. At 18 weeks, CHL and Chl had reduced the volume percent of liver occupied by GST placental form-positive foci by 74% (P < 0.001) and 77% (P < 0.001), respectively compared with control livers. CHL and Chl reduced the mean number of aberrant crypt foci per colon by 63% (P = 0.0026) and 75% (P = 0.0004), respectively. These results show Chl and CHL provide potent chemoprotection against early biochemical and late pathophysiological biomarkers of AFB1 carcinogenesis in the rat liver and colon.

Journal Article.  8012 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.