Journal Article

Hydroxyurea-induced global transcriptional suppression in mouse ES cells

Peng Cui, Qiang Lin, Chengqi Xin, Lu Han, Lili An, Yulan Wang, Zhishang Hu, Feng Ding, Lingfang Zhang, Songnian Hu, Haiying Hang and Jun Yu

in Carcinogenesis

Volume 31, issue 9, pages 1661-1668
Published in print September 2010 | ISSN: 0143-3334
Published online May 2010 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgq106
Hydroxyurea-induced global transcriptional suppression in mouse ES cells

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Hydroxyurea (HU), as a therapeutic medicine, has been extensively used clinically. To further survey molecular mechanisms of HU treatment, we analyzed global transcriptomic alteration of mouse ES cells in response to the treatment using high-throughput sequencing. We show that the global transcriptional activity is significantly suppressed as cells are exposed to HU treatment and alters multiple key cellular pathways, including cell cycle, apoptosis and DNAs. HU treatment also alters alternative splicing mechanisms and suppresses non-coding RNA expression. Our result provides novel clues for the understanding of how cells respond to HU and further suggests that high-throughput sequencing technology provides a powerful tool to study mechanisms of clinical drugs at the cellular level.

Journal Article.  6123 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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