Journal Article

Activation of Akt and MAPK pathways enhances the tumorigenicity of CD133+ primary colon cancer cells

Y. K. Wang, Y. L. Zhu, F. M. Qiu, T. Zhang, Z. G. Chen, S. Zheng and J. Huang

in Carcinogenesis

Volume 31, issue 8, pages 1376-1380
Published in print August 2010 | ISSN: 0143-3334
Published online June 2010 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgq120
Activation of Akt and MAPK pathways enhances the tumorigenicity of CD133+ primary colon cancer cells

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Cancer stem cells (CSCs) play an important role in carcinogenesis, resistance to treatment and may lead to cancer recurrence and metastasis. However, the molecular mechanism of CSC involved in these events needs to be further elucidated. In this study, CD133+ colon cancer cells were cultured, which showed CSC properties both in vitro and in vivo from metastatic tissue. Upstream molecules in Akt and mitogen-activated protein kinase (MAPK) pathways were preferentially expressed in these CD133+ cells, as revealed by a global gene chip. The kinase activities of Akt and extracellular signal-regulated kinase (Erk)1/2 were also significantly upregulated in CD133+ cells. In addition, the clonogenic growth of CD133+ cell was reduced greatly by inhibiting the activity of Akt and Erk1/2. The results revealed the Akt and MAPK pathways were involved in the tumorigenesis of CD133+ colon cancer cells, suggesting that molecules in these two pathways might be potential targets in the future therapy.

Journal Article.  3835 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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