Journal Article

<i>RUNX3</i> methylation and expression associated with advanced precancerous gastric lesions in a Chinese population

Wen-Qing Li, Kai-Feng Pan, Yang Zhang, Cai-Xuan Dong, Lian Zhang, Jun-Ling Ma, Tong Zhou, Ji-You Li and Wei-Cheng You

in Carcinogenesis

Volume 32, issue 3, pages 406-410
Published in print March 2011 | ISSN: 0143-3334
Published online December 2010 | e-ISSN: 1460-2180 | DOI: http://dx.doi.org/10.1093/carcin/bgq259
RUNX3 methylation and expression associated with advanced precancerous gastric lesions in a Chinese population

More Like This

Show all results sharing this subject:

  • Clinical Cytogenetics and Molecular Genetics

GO

Show Summary Details

Preview

Runt-related transcription factor 3 (RUNX3) is a tumor suppressor of gastric cancer. Our study aimed to investigate the correlation of RUNX3 methylation, expression and the risk of advanced gastric lesions, based on a high-risk population in Linqu County, Shandong Province, China. Methylation status of RUNX3 was determined by methylation-specific polymerase chain reaction, and expression was detected by immunohistochemical analysis in 1113 subjects with different gastric lesions. Results showed that the frequency of RUNX3 methylation was significantly increased in subjects with advanced gastric lesions. The odds ratios (ORs) were 2.09 [95% confidence interval (CI): 1.49–2.94] for intestinal metaplasia (IM), 3.22 (95% CI: 2.33–4.47) for indefinite dysplasia (Ind DYS) and 2.03 (95% CI: 1.23–3.37) for dysplasia (DYS) compared with superficial gastritis/chronic atrophic gastritis. Stratified analysis indicated that the frequency of RUNX3 methylation was higher in subjects with Helicobacter pylori infection (OR, 2.74; 95% CI: 2.00–3.76). Moreover, there was a reverse grade-response relationship between the level of RUNX3 expression and risk of gastric lesions. Among subjects with mild, moderate or heavy expression, the risk was decreased by 41, 59 or 80% for IM (Ptrend < 0.0001); 40, 64 or 74% for Ind DYS (Ptrend < 0.0001) and 28, 59 or 51% for DYS (Ptrend = 0.045), respectively. Furthermore, RUNX3 expression was negatively associated with increased frequency of RUNX3 methylation (OR, 0.76; 95% CI: 0.59–0.98). These findings suggest that RUNX3 may play important roles in the development of advanced gastric lesions.

Journal Article.  3720 words. 

Subjects: Clinical Cytogenetics and Molecular Genetics

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.