Journal Article

Perturbed replication induced genome wide or at common fragile sites is differently managed in the absence of WRN

Ivana Murfuni, Anita De Santis, Maurizio Federico, Margherita Bignami, Pietro Pichierri and Annapaola Franchitto

in Carcinogenesis

Volume 33, issue 9, pages 1655-1663
Published in print September 2012 | ISSN: 0143-3334
Published online June 2012 | e-ISSN: 1460-2180 | DOI:
Perturbed replication induced genome wide or at common fragile sites is differently managed in the absence of WRN

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The Werner syndrome protein (WRN) is a member of the RecQ helicase family. Loss of WRN results in a human disease, the Werner syndrome (WS), characterized by high genomic instability, elevated cancer risk and premature aging. WRN is crucial for the recovery of stalled replication forks and possesses both helicase and exonuclease enzymatic activities of uncertain biological significance. Previous work revealed that WRN promotes formation of MUS81-dependent double strand breaks (DSBs) at HU-induced stalled forks, allowing replication restart at the expense of chromosome stability. Here, using cells expressing the helicase- or exonuclease-dead WRN mutant, we show that both activities of WRN are required to prevent MUS81-dependent breakage after HU-induced replication arrest. Moreover, we provide evidence that, in WS cells, DSBs generated by MUS81 do not require RAD51 activity for their formation. Surprisingly, when replication is specifically perturbed at common fragile sites (CFS) by aphidicolin, WRN limits accumulation of ssDNA gaps and no MUS81-dependent DSBs are detected. However, in both cases, RAD51 is essential to ensure viability of WS cells, although by different mechanisms. Thus, the role of WRN in response to perturbation of replication along CFS is functionally distinct from that carried out at stalled forks genome wide. Our results contribute to unveil two different mechanisms used by the cell to overcome the absence of WRN.

Journal Article.  6804 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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