Journal Article

Lipid peroxidation-induced DNA adducts in human gastric mucosa

Tomonari Matsuda, Hong Tao, Masanori Goto, Hidetaka Yamada, Masaya Suzuki, Yijia Wu, Nong Xiao, Qiong He, Wenwen Guo, Zhenming Cai, Nobuya Kurabe, Keiko Ishino, Yoshitaka Matsushima, Kazuya Shinmura, Hiroyuki Konno, Masato Maekawa, Yaping Wang and Haruhiko Sugimura

in Carcinogenesis

Volume 34, issue 1, pages 121-127
Published in print January 2013 | ISSN: 0143-3334
Published online October 2012 | e-ISSN: 1460-2180 | DOI:
Lipid peroxidation-induced DNA adducts in human gastric mucosa

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DNA adducts are a major cause of DNA mutation and DNA mutation-related diseases, but the simultaneous identification of multiple DNA adducts has been a challenge for a decade. An adductome approach using consecutive liquid chromatography and double mass spectrometry after micrococcal nuclease treatment has paved the way to demonstrations of numerous DNA adducts in a single experiment and is expected to contribute to the comprehensive understanding of overall environmental and endogenous exposures to possible mutagens in individuals. In this report, we applied an adductome approach to gastric mucosa samples taken at the time of a gastrectomy for gastric cancer in Lujiang, China, and in Hamamatsu, Japan. Seven lipid peroxidation-related DNA adducts [1,N6-etheno-2′-deoxyadenosine, butanone-etheno-2′-deoxycytidine (BɛdC), butanone-etheno-2′-deoxy-5-methylcytidine, butanone-etheno-2′-deoxyadenosine (BɛdA), heptanone-etheno-2′-deoxycytidine, heptanone-etheno-2′-deoxyadenosine (HɛdA) and heptanone-etheno- 2′-deoxyguanosine] were identified in a total of 22 gastric mucosa samples. The levels of these adducts ranged from 0 to 30 000 per 109 bases. Although the presence of Helicobacter pylori DNA in the mucosa was not related to these adducts level, the levels of BɛdC, BɛdA and HɛdA were higher in the Japanese gastric mucosa samples. The profiles of these 7 adduct levels among the 21 cases were capable of discriminating between the possible origins (China or Japan) of the gastric mucosa samples. Our report is the first demonstration of lipid peroxidation-related DNA adducts in the human stomach, and these observations warrant further investigation in the context of the significance of DNA adducts in human gastric carcinogenesis.

Journal Article.  4212 words.  Illustrated.

Subjects: Clinical Cytogenetics and Molecular Genetics

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