Journal Article

Immunocytochemistry of Glutathione <i>S</i>-Transferase in Taste Bud Cells of Rat Circumvallate and Foliate Papillae

Tomoko Nishino, Hideaki Kudo, Yoshiaki Doi, Masanobu Maeda, Kunshige Hamasaki, Miyako Morita and Sunao Fujimoto

in Chemical Senses

Volume 26, issue 2, pages 179-188
Published in print February 2001 | ISSN: 0379-864X
Published online February 2001 | e-ISSN: 1464-3553 | DOI:
Immunocytochemistry of Glutathione S-Transferase in Taste Bud Cells of Rat Circumvallate and Foliate Papillae

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Immunocytochemistry was used to investigate the distribution of cells reacting with specific antibodies against glutathione S-transferase (GST) μ and π in rat circumvallate and foliate taste buds; the findings were confirmed by Western blotting. Double immunofluorescence staining for protein gene product (PGP) 9.5 and GST subunits allowed the classification of taste bud cells of both papillae into: (i) cells immunoreactive to either PGP 9.5 or GST subunit antibody; (ii) cells immunoreactive to both antibodies; and (iii) cells that did not react with either of these antibodies. Immunoelectron microscopy revealed that most GST subunit-immunoreactive cells seemed to be either type II or type III cells based on their ultrastructure. Since PGP 9.5 is now widely used as a marker for type III cells in mammalian taste buds, it seems reasonable to believe that most GST subunit-immunoreactive cells are type II cells. Whether cells immunoreactive for both PGP 9.5 and GST subunits consitute a small subpopulation of type III cells or whether they are intermediate forms between type II and III cells is under investigation. No type I cells reacted with antibodies against GST subunits in the present study. GST subunits in taste bud cells may participate in xenobiotic metabolism of certain substances exposed to taste pits, as already shown for olfactory epithelium.

Journal Article.  4715 words.  Illustrated.

Subjects: Molecular and Cell Biology

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