Journal Article

Analysis of Steroids by Capillary Supercritical Fluid Chromatography with Flame-lonization and Electron-Capture Detectors

C. Baiocchi, D. Giacosa, M.A. Roggero and E. Marengo

in Journal of Chromatographic Science

Volume 34, issue 9, pages 399-404
Published in print September 1996 | ISSN: 0021-9665
Published online September 1996 | e-ISSN: 1945-239X | DOI: http://dx.doi.org/10.1093/chromsci/34.9.399
Analysis of Steroids by Capillary Supercritical Fluid Chromatography with Flame-lonization and Electron-Capture Detectors

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The separation and determination of several steroidal substances are achieved with capillary supercritical fluid chromatography by using supercritical CO2 as mobile phase. Different stationary phases are used to compare chromatographic results, optimize the separation, and obtain information about the chromatographic behavior of the substances examined. Unexpected elution orders and problems of poor sensitivity of the flame-ionization detector towards some compounds are discussed. Alternative chromatographic conditions are explored: an electron-capture detector is used with suitably optimized pressure gradients in an attempt to overcome these limitations.

Journal Article.  0 words. 

Subjects: Chemistry

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