Journal Article

Quantitative Determination of the Loop Diuretic Bumetanide in Urine and Pharmaceuticals by High-Performance Liquid Chromatography with Amperometric Detection

M.J. Legorburu, R.M. Alonso, R.M. Jiménez and E. Ortiz

in Journal of Chromatographic Science

Volume 39, issue 10, pages 425-430
Published in print October 2001 | ISSN: 0021-9665
Published online October 2001 | e-ISSN: 1945-239X | DOI: http://dx.doi.org/10.1093/chromsci/39.10.425
Quantitative Determination of the Loop Diuretic Bumetanide in Urine and Pharmaceuticals by High-Performance Liquid Chromatography with Amperometric Detection

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A high-performance liquid chromatographic method with amperometric detection has been developed for the determination of the diuretic bumetanide using a µBondapak C18 column. The mobile phase consists of a 50:50 acetonitrile-water mixture containing 5mM KH2PO4-K2HPO4 (pH 4.0). The compound is monitored at +1350 mV with an amperometric detector equipped with a glassy carbon working electrode. A liquid-liquid or solid-liquid extraction is done prior to chromatographic analysis in order to avoid the interferences found in the urine matrix. The percentages of recovery obtained are 71% ± 1% for liquid-liquid extraction and 84.2% ± 0.7% for solid-liquid extraction. The method developed has a linear concentration range from 50 to 499 ng/mL with a reproducibility in terms of relative standard deviation of 1.73% and 3.85% for a concentration level of 70 ng/mL and 237 ng/mL, respectively, and a detection limit of 0.25 ng/mL (3:1 signal-to-noise ratio). The method is applied to the determination of bumetanide in pharmaceutical formulations and urine obtained from hypertensive patients and healthy volunteers after the ingestion of a therapeutic dose of Fordiuran (1 mg bumetanide).

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Subjects: Chemistry

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