Journal Article

Multiple Peak Formation of Avidin on an Iminobiotin Affinity Support

Yan Wang and Tingyu Li

in Journal of Chromatographic Science

Volume 42, issue 2, pages 85-87
Published in print February 2004 | ISSN: 0021-9665
Published online February 2004 | e-ISSN: 1945-239X | DOI: http://dx.doi.org/10.1093/chromsci/42.2.85
Multiple Peak Formation of Avidin on an Iminobiotin Affinity Support

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When pure avidin is chromatographed on a homemade iminobiotin column, two distinct peaks are observed using a gradient solvent system starting with a pH 9 buffer. Proteins in both peaks are shown to be identical by nondenatured polyacrylamide gel electrophoretic experiment. The weak interaction of the protein with the stationary phase at pH 9 could cause this unusual behavior.

Journal Article.  0 words. 

Subjects: Chemistry

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