Journal Article

Preparative High-Performance Liquid Chromatographic Separation of Naphthodianthrones from St. John's Wort

S. Puri, G. Handa, A.K. Kalsotra, V.K. Gupta, A.S. Shawl, O.P. Suri and G.N. Qazi

in Journal of Chromatographic Science

Volume 44, issue 4, pages 177-180
Published in print April 2006 | ISSN: 0021-9665
Published online April 2006 | e-ISSN: 1945-239X | DOI: https://dx.doi.org/10.1093/chromsci/44.4.177

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St. John's Wort (Hypericum perforatum), a perennial flowering plant, has been used medicinally for thousands of years and has most recently been identified as an effective treatment for mild to moderate depression and neuralgic disorders. This work presents a procedure for the isolation of naphthodianthrones from St. John's Wort by an accelerated extraction and separation of marker compounds by preparative high-performance liquid chromatography (HPLC) with photodiode array detection. The accelerated extraction method minimizes the extraction time and increases the yield, and the marker compounds obtained by preparative HPLC are of 98% purity. The compounds are characterized by liquid chromatography-mass spectrometry (electrospray ionization) and NMR spectra.

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Subjects: Chemistry