Journal Article

Determination of Ethambutol Hydrochloride in the Combination Tablets by Precolumn Derivatization

Ming Yan, Tao Guo, Hongtao Song, Qingchun Zhao and Yin Sui

in Journal of Chromatographic Science

Volume 45, issue 5, pages 269-272
Published in print May 2007 | ISSN: 0021-9665
Published online May 2007 | e-ISSN: 1945-239X | DOI: http://dx.doi.org/10.1093/chromsci/45.5.269
Determination of Ethambutol Hydrochloride in the Combination Tablets by Precolumn Derivatization

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A new high-performance liquid chromatography method for the quantitative determination of ethambutol hydrochloride in combination tablets is presented. Ethambutol is derivatized with phenylethylisocyanatate at room temperature (22 ± 2°C) for 5 min. Separation is performed by a C18 column using methanol-water-glacial acetic acid (70:30:0.2, v/v/v) as the mobile phase. The method is linear for drug concentrations in the range of 20–120 µg/mL (r = 0.9995). The intra- and inter-day precisions are lower than 1.46% and 2.22%, respectively. The average recovery of the samples at three levels is 99.8%. The results show that derivatization of ethambutol is stable at 30°C for 24 h. This method is simple, rapid, and stable in the presence of common excipients and antituberculosis drugs in the tablets.

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Subjects: Chemistry

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