Journal Article

Capillary Gas Chromatographic Determination of Methylglyoxal from Serum of Diabetic Patients by Precolumn Derivatization Using Meso-Stilbenediamine as Derivatizing Reagent

A.J. Kandhro, M. Aslam Mirza and M.Y. Khuhawar

in Journal of Chromatographic Science

Volume 46, issue 6, pages 539-543
Published in print July 2008 | ISSN: 0021-9665
Published online July 2008 | e-ISSN: 1945-239X | DOI: http://dx.doi.org/10.1093/chromsci/46.6.539
Capillary Gas Chromatographic Determination of Methylglyoxal from Serum of Diabetic Patients by Precolumn Derivatization Using Meso-Stilbenediamine as Derivatizing Reagent

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Stilbenediamine is used as derivatizing reagent for methylglyoxal (MGo) and dimethylglyoxal for the gas chromatographic (GC) determination of MGo from the serum of diabetic patients and healthy volunteers. The derivatization is obtained at pH 3. GC elution and separation are carried out on an HP5 column (30 m × 0.32 mm i.d.) at column temperature 150°C with a programmed heating rate of 50°C/min up to 250°C, and a total run time of 7 min. The nitrogen flow rate is 5 mL/min and detection is carried out by flame ionization detection. The linear calibration curves are obtained with a range of 0.076–0.760 µg/mL and the detection limit is 25 ng/mL MGo. The amounts of MGo found in the serum of healthy volunteers and diabetic patients are 0.025–0.065 µg/mL and 0.115–0.228 µg/mL, with coefficient of variation 1.3–3.1% and 1.4–3.3%.

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Subjects: Chemistry

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