Journal Article

Determination of Para Red, Sudan Dyes, Canthaxanthin, and Astaxanthin in Animal Feeds Using UPLC

Xiaolin Hou, Yonggang Li, Guojuan Wu, Lei Wang, Miao Hong and Yongnin Wu

in Journal of Chromatographic Science

Volume 48, issue 1, pages 22-25
Published in print January 2010 | ISSN: 0021-9665
Published online January 2010 | e-ISSN: 1945-239X | DOI: https://dx.doi.org/10.1093/chromsci/48.1.22
Determination of Para Red, Sudan Dyes, Canthaxanthin, and Astaxanthin in Animal Feeds Using UPLC

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A simple high-performance liquid chromatography method was developed for quantitative determination of para red, Sudan I, Sudan II, Sudan III, Sudan IV, canthaxanthin, and astaxanthin in feedstuff. The sample was extracted using acetontrile and cleaned up on a C18 SPE column. The residues were analyzed using ultraperformance liquid chromatography coupled to a diode array detector at 500 nm. The mobile phase was acetonitrile-formic acid-water with a gradient elution condition. The external standard curves were calibrated. The mean recoveries of the seven colorants were 62.7–91.0% with relative standard deviation 2.6–10.4% (intra-day) and 4.0–13.2% (inter-day). The detection limits were in the range of 0.006-0.02 mg/kg.

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Subjects: Chemistry

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