Journal Article

Rhodamine B as Ligand for Affinity Chromatography: Chromatographic Studies on Derivatized Beaded Cellulose

Andreia Silva, Renato E.F. Boto, Cândida T. Tomaz, Joāo A. Queiroz and Paulo Almeida

in Journal of Chromatographic Science

Volume 48, issue 3, pages 240-244
Published in print March 2010 | ISSN: 0021-9665
Published online March 2010 | e-ISSN: 1945-239X | DOI: http://dx.doi.org/10.1093/chromsci/48.3.240
Rhodamine B as Ligand for Affinity Chromatography: Chromatographic Studies on Derivatized Beaded Cellulose

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Rhodamine B (RB) post-grafted onto beaded cellulose by a curing method is used as a biomimetic ligand in dye affinity chromatography. The grafted materials obtained are qualitatively characterized by scanning electron microscope and fourier transformed infrared spectroscopy. An amount of 76.79 mmol RB/g dyed cellulose is determined by elemental analysis. The RB affinity interaction with the trypsin, α-chymotrypsin, and BSA (bovine serum albumine) is analyzed using different mobile phase composition. The results show a selective separation of a mixture of BSA and trypsin into two single peaks by step elution with 1.75 M, 0.5 M, and 0 M ammonium sulphate in the eluent buffer. A good reproducibility of the retention time is obtained for these proteins in the mixture with typical values of 8.0 ± 0.2 min for BSA and 20.0 ± 0.2 min for trypsin, showing a possible application in the purification of samples with different composition.

Journal Article.  0 words. 

Subjects: Chemistry

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