Journal Article

Characterization of Long cDNA Clones from Human Adult Spleen. II. The Complete Sequences of 81 cDNA Clones

Hiroyuki Jikuya, Jun Takano, Reiko Kikuno, Makoto Hirosawa, Takahiro Nagase, Nobuo Nomura and Osamu Ohara

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 10, issue 1, pages 49-57
Published in print January 2003 | ISSN: 1340-2838
Published online January 2003 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/10.1.49

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To accumulate information on the coding sequences (CDSs) of unidentified genes, we have conducted a sequencing project of human long cDNA clones. Both the end sequences of approximately 10,000 cDNA clones from two size-fractionated human spleen cDNA libraries (average sizes of 4.5 kb and 5.6 kb) were determined by single-pass sequencing to select cDNAs with unidentified sequences. We herein present the entire sequences of 81 cDNA clones, most of which were selected by two approaches based on their protein-coding potentialities in silico: Fifty-eight cDNA clones were selected as those having protein-coding potentialities at the 5′-end of single-pass sequences by applying the GeneMark analysis; and 20 cDNA clones were selected as those expected to encode proteins larger than 100 amino acid residues by analysis of the human genome sequences flanked by both the end sequences of cDNAs using the GENSCAN gene prediction program. In addition to these newly identified cDNAs, three cDNA clones were isolated by colony hybridization experiments using probes corresponding to known gene sequences since these cDNAs are likely to contain considerable amounts of new information regarding the genes already annotated. The sequence data indicated that the average sizes of the inserts and corresponding CDSs of cDNA clones analyzed here were 5.0 kb and 2.0 kb (670 amino acid residues), respectively. From the results of homology and motif searches against the public databases, functional categories of the 29 predicted gene products could be assigned; 86% of these predicted gene products (25 gene products) were classified into proteins relating to cell signaling/communication, nucleic acid management, and cell structure/motility.

Keywords: long cDNA; single-pass sequence; cDNA sequencing; spleen

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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