Journal Article

<i>Peas-Mea1-Ppp2r5d</i> Overlapping Gene Complex: a Transposon Mediated-gene Formation in Mammals

Yasuhide Ohinata, Shizuyo Sutou and Youji Mttsui

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 10, issue 2, pages 79-84
Published in print January 2003 | ISSN: 1340-2838
Published online January 2003 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/10.2.79

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Human and mouse MEA1/Mea1 is flanked by two overlapping genes, a novel PEAS/Peas in a head-to-head orientation and PPP2R5D/Ppp2r5d in a tail-to-tail orientation making a Peas-Mea1-Ppp2r5d overlapping gene complex (PMP-complex). Genomic zoo blot analyses and database searching revealed that Mea1 exists only in mammals, while Peas and Ppp2r5d are conserved in eukaryotes. Mea1 and Peas are transcribed from a testis-expressed bidirectional promoter. Mea1-Ppp2r5d overlapping segment (MPOS) contains polyadenylation signals for both genes and shows marked conservation throughout mammals. Furthermore, the MPOS occupies 3′-region of transcripts of both genes is expected to form a clover-like intramolecular structure. Mouse genomic library Screening and database searches identified two MPOS-derived sequences in Odf2 gene and RP23-86H7 cosmid clone, respectively, in which MPOS might be a core segment for the retropositions. Thus, a key role of MPOS, a short transposable element containing polyadenylation signals on both strands, in the formation of the Mea1 during mammalian evolution is suggested.

Keywords: overlapping genes; Peas; Mea1; Ppp2r5d; transposable element

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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