Journal Article

Structural Analysis of a <i>Lotus japonicus</i> Genome. IV. Sequence Features and Mapping of Seventy-three TAC Clones Which Cover the 7.5 Mb Regions of the Genome

Erika Asamizu, Tomohiko Kato, Shusei Sato, Yasukazu Nakamura, Takakazu Kaneko and Satoshi Tabata

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 10, issue 3, pages 115-122
Published in print January 2003 | ISSN: 1340-2838
Published online January 2003 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/10.3.115

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Using the sequence information of expressed sequences tags (ESTs), cDNAs and genes from Lotus japonicus and other legumes, 73 TAC (transformation-competent arti.cial chromosomes) clones were selected from a genomic library of L. japonicus accession MG-20, and their nucleotide sequences were determined. The length of the DNA sequenced in this study was 7,455,959 bp, and the total length the DNA regions sequenced so far is 26,167,443 bp together with the nucleotide sequences of 183 TAC clones previously reported. By similarity searches against the sequences in protein and EST databases and prediction by computer programs, a total of 699 potential protein-encoding genes with known or predicted functions, 163 gene segments and 267 pseudogenes were assigned to the newly sequenced regions. Based on the nucleotide sequences of the clones, simple sequence repeat length polymorphism (SSLP) or derived cleaved amplified polymorphic sequence (dCAPS) markers were generated, and each clone was located onto the linkage map of two accessions of L. japonicus, Gifu B-129 and Miyakojima MG-20. The sequence data, gene information and mapping information are available through the World Wide Web at .

Keywords: Lotus japonicus; genomic sequence; TAC genomic library; gene prediction; linkage mapping

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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