Journal Article

Identification of a Human cDNA Sequence Which Encodes a Novel Membrane-associated Protein Containing a Zinc Metalloprotease Motif

Ying-Chun Bao, Hiromichi Tsuruga, Momoki Hirai, Kazuki Yasuda, Norihide Yokoi, Toshio Kitamura and Hidetoshi Kumagai

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 10, issue 3, pages 123-128
Published in print January 2003 | ISSN: 1340-2838
Published online January 2003 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/10.3.123

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We report the cloning and characterization of a human cDNA predicted to encode a novel hydrophobic protein containing four transmembrane domains and a zinc metalloprotease motif, HEXXH, between the third and fourth transmembrane domains, and have named the molecule metalloprotease-related protein-1 (MPRP-1). The MPRP-1 gene was localized to chromosome 1-p32.3 by radiation hybrid mapping, and Northern blot analysis revealed expression in many organs, with strong expression in the heart, skeletal muscle, kidney and liver. Immunohistochemical analyisis showed that MPRP-1 was localized in the endoplasmic reticulum (ER), and not in the Golgi compartment. Fragments of DNA encoding a segment homologous to the HEXXH motif of MPRP-1 are widely found in bacteria, yeast, plants, and animals. These results suggest that the MPRP-1 may have highly conserved functions, such as in intracellular proteolytic processing in the ER.

Keywords: metalloprotease; membrane protein; endoplasmic reticulum

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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