Journal Article

Gene Expression Profiling Identifies a Set of Transcripts that are Up-Regulated in Human Testicular Seminoma

Shigeyuki Yamada, Kazuyoshi Kohu, Tomohiko Ishii, Shigeru Ishidoya, Masayoshi Hiramatsu, Satoru Kanto, Atsushi Fukuzaki, Yutsu Adachi, Mareyuki Endoh, Takuya Moriya, Hiroki Sasaki, Masanobu Satake and Yoichi Arai

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 11, issue 5, pages 335-344
Published in print January 2004 | ISSN: 1340-2838
Published online January 2004 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/11.5.335

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Seminoma constitutes one subtype of human testicular germ cell tumors and is uniformly composed of cells that are morphologically similar to the primordial germ cells and/or the cells in the carcinoma in situ. We performed a genome-wide exploration of the genes that are specifically up-regulated in seminoma by oligonucleotide-based microarray analysis. This revealed 106 genes that are significantly and consistently up-regulated in the seminomas compared to the adjacent normal tissues of the testes. The microarray data were validated by semi-quantitative RT-PCR analysis. Of the 106 genes, 42 mapped to a small number of specific chromosomal regions, namely, 1q21, 2p23, 6p21-22, 7p14-15, 12p11, 12p13, 12q13-14 and 22q12-13. This list of up-regulated genes may be useful in identifying the causative oncogene(s) and/or the origin of seminoma. Furthermore, immunohistochemical analysis revealed that the seminoma cells specifically expressed the six gene products that were selected randomly from the list. These proteins include CCND2 and DNMT3A and may be useful as molecular pathological markers of seminoma.

Keywords: testicular seminoma; gene expression; microarray analysis; immunohistochemistry

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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