Journal Article

Large-Scale Identification and Mapping of Nuclear Matrix-Attachment Regions in the Distal Imprinted Domain of Mouse Chromosome 7

Wahyu Purbowasito, Chikako Suda, Takaaki Yokomine, Mohamad Zubair, Takashi Sado, Ken Tsutsui and Hiroyuki Sasaki

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 11, issue 6, pages 391-407
Published in print January 2004 | ISSN: 1340-2838
Published online January 2004 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/11.6.391

Show Summary Details

Preview

Mammalian imprinted genes, which are expressed from only one of the parental alleles, have a tendency to form clusters and are regulated by long-range mechanisms. Nuclear matrix-attachment regions (MARs), the anchor points of loop domains, are involved in coordination of gene expression and could play a role in regulation of imprinted domains. We have identified and mapped a total of 52 MARs in a 1-Mb imprinted domain on mouse distal chromosome 7 using our cosmid contigs and an in vitro MAR assay. We find two MAR clusters (comprising 20 and 19 MARs), one of which is mapped in the Th-Ins2 intergenic region, coincident with the boundary between the two imprinted subdomains. However, the imprinted/nonimprinted boundaries are not associated with a MAR. Based on the sequence information, we find that many of the MARs are rich in long interspersed nuclear elements. In addition, comparisons of the results obtained with several MAR-prediction software programs reveal good performance of ChrClass in terms of both sensitivity and specificity. This study presents the first large-scale mapping of MARs in an imprinted domain and provides a platform for understanding the roles of MARs in imprinting.

Keywords: genomic imprinting; cosmid contig; nuclear-matrix attachment region; long interspersed nuclear element

Journal Article.  0 words. 

Subjects: Genetics and Genomics

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.