Journal Article

<i>Lotus burttii</i> Takes a Position of the Third Corner in the <i>Lotus</i> Molecular Genetics Triangle

Masayoshi Kawaguchi, Andrea Pedrosa-Harand, Koji Yano, Makoto Hayashi, Yoshikatsu Murooka, Katsuharu Saito, Toshiyuki Nagata, Kiyoshi Namai, Hiroshi Nishida, Daisuke Shibata, Shusei Sato, Satoshi Tabata, Masaki Hayashi, Kyuya Harada, Niels Sandal, Jens Stougaard, Andreas Bachmair and William F. Grant

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 12, issue 1, pages 69-77
Published in print January 2005 | ISSN: 1340-2838
Published online January 2005 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/12.1.69

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In order to consolidate molecular genetic system in Lotus japonicus and to further access the biological diversity in Lotea, we introduce here Lotus burttii B-303 derived from West Pakistan as the third crossing partner of the Gifu ecotype (B-129-S9) for a genetic analysis. L. burttii is a relatively small and early flowering plant with non-shattering behavior. The general chromosome morphology is very similar to Gifu, and fluorescence in situ hybridization (FISH) analysis revealed that the short arm of chromosome 1 in L. burttii is comparable to that of Gifu, indicating that the translocation event involving chromosomes 1 and 2, which was observed in L. japonicus Miyakojima MG-20, is not present in L. burttii. In addition L. burttii has a higher level of DNA polymorphism compared to Gifu and MG-20 enabling design of codominant markers such as SSR, CAPS and dCAPS. Using an F2 population from a cross between Gifu and L. burttii, codominant makers that co-segregated at the translocation site could be expanded. In order to normalize the genetic background, L. burttii was inbred for nine generations and the germplasm L. burttii B-303-S9 was established.

Keywords: Lotus burttii; Lotus japonicus; molecular genetics; fluorescence in situ hybridization (FISH); crossing partner

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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