Journal Article

Structure and Expression of the Tobacco Nuclear Gene Encoding RNA-binding Protein RZ-1: The Existence of an Intron in the 3′-Untranslated Region

Shigeru Hanano, Mamoru Sugita and Masahiro Sugiura

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 3, issue 2, pages 65-71
Published in print January 1996 | ISSN: 1340-2838
Published online January 1996 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/3.2.65

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We have previously characterized a tobacco cDNA encoding a novel type RNA-binding protein (RZ-1), which contains a zinc finger motif in addition to a consensus sequence-type RNA-binding domain and is localized in the nucleus. Here we isolated its genomic clone from a Nicotiana sylvestris genomic library. Southern blot analysis suggested that RZ-1 is coded for by a single locus per haploid genome. Comparison of the cDNA and genomic sequences indicated that the RZ-1 gene contains two introns, one in the coding region and another in the 3′-untranslated region. RT-PCR and ribonuclease protection analyses showed that splicing of RZ-1 pre-mRNA occurs efficiently. The RZ-1 protein is actively synthesized in rapidly dividing tobacco cells, as demonstrated by immunoblot analysis.

Keywords: RNA-binding protein; tobacco; intron; 3′-untranslated region; splicing

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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