Journal Article

The Effect of the<i>crp</i> Genotypes on the Transformation Efficiency in <i>Escherichia coli</i>

Aiko Umemoto, Masayuki Morita, Naoki Nakazono and Yoshinobu Sugino

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 3, issue 2, pages 93-94
Published in print January 1996 | ISSN: 1340-2838
Published online January 1996 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/3.2.93

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We have found that a significant difference exists in transformation efficiency between the crp+/crp isogenic pair of strains of Escherichia coli, with the efficiency being much higher in crp than in crp+. The ratio of transformation efficiency between crp+ and crp strains depends very little on the plasmid size. This observation suggests that the difference of the transformation efficiency is due to mechanisms other than a crp-regulated endonuclease. The crp gene is one of the first specific genes that have been shown to affect transformation efficiency.

Keywords: crp; transformation efficiency; electroporation; calcium treatment; transfection

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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