Journal Article

A Panel of Radiation Hybrids Defining the 7q31-q32 Region of Human Chromosome 7

Kazuhiko Nakabayashi, Toshihiko Ogata, Michihiko Fujii, Ei-ichi Takahashi, Hideki Ogino, Eriko Michishita, Stephen W. Scherer, Lap-Chee Tsui and Dai Ayusawa

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 3, issue 3, pages 181-183
Published in print January 1996 | ISSN: 1340-2838
Published online January 1996 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/3.3.181

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Mouse A9 cells containing human chromosome 7 tagged with pSV2neo were irradiated with X-rays and fused to A9 cells to isolate G418-resistant clones. From these clones, we selected radiation hybrids that contained 10–40 Mb of human DNA apparently at a single site of their genome by FISH analysis using human repetitive sequences as a probe. Then we made a panel of hybrids that contained various fragments of the 7q31-q32 region and cover its entire region altogether by PCR with STS markers of human chromosome 7. This panel is useful in chromosome transfer experiments since the dominant selective marker neo gene is attached to human DNA.

Keywords: chromosome 7; sequence tagged sites (STS); radiation hybrid

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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