Journal Article

Transcript Map of the Human Chromosome 4p16.3 Consisting of 627 cDNA Clones Derived from 1 Mb of the Huntington's Disease Locus

Shinji Hadano, Yoshikazu Ishida, Hitoshi Tomiyasu, Kenji Yamamoto, Gillian P. Bates and Joh-E Ikeda

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 3, issue 4, pages 239-255
Published in print January 1996 | ISSN: 1340-2838
Published online January 1996 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/3.4.239

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Six hundred and twenty-seven cDNA clones from human brain cDNA libraries were characterized and integrated into a transcript map of the 1-Mb region on human chromosome 4p16.3 containing the Huntington's disease (HD) gene. Six hundred and seventy-two cDNA clones were obtained by a direct screening of the cDNA libraries, probing with pools of single copy microclones generated from the HD region specific yeast artificial chromosome (YAC)-DNA. So far, 93% of the obtained clones (627 cDNA clones) have been mapped onto the 1-Mb HD gene region by hybridization with HD region-specific cosmid, P1 and YAC clones. DNA sequence and expression analyses revealed that several cDNA clones might encode novel genes, some of which are situated within or close to the IT15, IT11, and α-adducin (ADD1) gene region, suggesting the presence of the overlapping genes in this region. This collection of cDNA clones will greatly facilitate the construction of the complete map of the transcripts in the HD region.

Keywords: Huntington's disease; human chromosome 4p16.3; transcript map; cDNA

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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