Journal Article

Isolation and Analysis of a Novel Gene, <i>HXC-26</i>, Adjacent to the <i>rab</i> GDP Dissociation Inhibitor Gene Located at Human Chromosome Xq28 Region

Atsushi Toyoda, Tsuyoshi Sakai, Yoshikazu Sugiyama, Jun Kusuda, Katsuyuki Hashimoto and Hidekatsu Maeda

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 3, issue 5, pages 337-340
Published in print January 1996 | ISSN: 1340-2838
Published online January 1996 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/3.5.337

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We screened potential promoter regions from NotI-linking cosmid clones mapped on human chromosome Xq28 region with our constructed trapping vector and isolated six fragments containing transcription activity. Using one of the obtained fragments as a probe, a novel gene was isolated by screening a human skeletal muscle cDNA library. The isolated cDNA, termed HXC-26, contained an open reading frame of 975 nucleotides encoding 325 amino acids (38,848 Da). The HXC-26 gene was composed of 13 exons that span ∼8 kb. Several potential GC boxes were found in the putative promoter region, but no typical TATA box. The HXC-26 gene associated with a CpG island was located adjacent to the rab GDP dissociation inhibitor (GDI) gene.

Keywords: cDNA cloning; human chromosome Xq28; HXC-26

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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