Journal Article

A 570-kb DNA Sequence of the <i>Escherichia coli</i> K-12 Genome Corresponding to the 28.0–40.1 min Region on the Linkage Map

Hiroji Aiba, Tomoya Baba, Kouji Hayashi, Toshifumi Inada, Katumi Isono, Takeshi Itoh, Hiroaki Kasai, Kaoru Kashimoto, Shigenobu Kimura, Madoka Kitakawa, Masanari Kitagawa, Kozo Makino, Takeyoshi Miki, Kiyoshi Mizobuchi, Hirotada Mori, Tomoko Mori, Kouji Motomura, Shinsuke Nakade, Yoshikazu Nakamura, Hiroko Nashimoto, Yoshitaka Nishio, Taku Oshima, Noriko Saito, Gen-ichi Sampei, Yasushi Seki, Suharnan Sivasundaram, Hideaki Tagami, Jun-ichi Takeda, Keiko Takemoto, Yasushi Takeuchi, Chieko Wada, Yoshihiro Yamamoto and Takashi Horiuchi

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 3, issue 6, pages 363-377
Published in print January 1996 | ISSN: 1340-2838
Published online January 1996 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/3.6.363

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The 569,750 base pair sequence corresponding to the 28.0–40.1 min region on the genetic map of Escherichia coli K-12 (W3110) was determined. This region includes the replication terminus region and contained at least 549 potential open reading frames. Among them, 160 (29%) were previously reported, 174 (32%) were homologous to other known genes, 102 (18%) were identical or similar to hypothetical genes registered in databases, and the remaining 113 (21%) did not show a significant similarity to any other gene. Of interest was the finding of a large number of genes and gene clusters in andnear the replication termination region which had been thought to be genetically silent. Those includeda cluster of genes for fatty acid β-oxidation, the third copy of the pot (spermidine/putrescine transport system) gene cluster, the second dpp (dipeptide transport system) operon, the second dsm (anaerobic dimethyl sulfoxide reductase) operon, a cluster of fim (fimbrial) genes anda DNA helicase-like gene with a high molecular weight. In addition, we found the dnaC- and dnaT-like genes in the cryptic prophage, Rac, anda number of genes originated probably from plasmids.

Keywords: Escherichia coli K12; genome sequence; ORF analysis; replication termination region

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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