Journal Article

Physical Mapping of Rice Chromosomes 8 and 9 with YAC Clones

Baltazar A. Antonio, Makiko Emoto, Jianzhong Wu, Ikuo Ashikawa, Yosuke Umehara, Nori Kurata and Takuji Sasaki

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 3, issue 6, pages 393-400
Published in print January 1996 | ISSN: 1340-2838
Published online January 1996 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/3.6.393

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First efforts for physical mapping of rice chromosomes 8 and 9 were carried out by ordering YAC clones of a rice genomic DNA library covering six genome equivalents with mapped DNA markers. A total of 79 and 74 markers from chromosomes 8 and 9, respectively, were analyzed by YAC colony and Southern hybridization using RFLP markers of cDNA and genomic clones, and by polymerase chain reaction (PCR) screening using PCR-derived and sequence-tagged site (STS) markers. As a result, 252 YAC clones were confirmed to contain the mapped DNA fragments on both chromosomes. A contig map was constructed by ordering these YAC clones and about 53% and 43% genome coverage was obtained for chromosomes 8 and 9, respectively, assuming a YAC clone size of 350 kb and overlap between neighboring YACs of 50%. A continuous array of YAC clones with minimum overlap gave a total size of 18.9 Mb for chromosome 8 and 15.6 Mb for chromosome 9, which are close to previous estimates. These contig maps may provide valuable information that can be useful in understanding chromosome structure and isolating specific genes by map-based cloning.

Keywords: physical map; chromosome 8; chromosome 9; YAC contigs; YAC islands

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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