Journal Article

Breakpoint Junction of Interstitial Homozygous Deletion at Chromosome 2q33 in a Small Cell Lung Carcinoma

Takashi Kohno, Toshiyuki Otsuka, Johji Inazawa, Tatsuo Abe and Jun Yokota

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 3, issue 6, pages 421-424
Published in print January 1996 | ISSN: 1340-2838
Published online January 1996 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/3.6.421

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We have characterized the breakpoint junction of the homozygous deletion at chromosome 2q33 in a small cell lung carcinoma cell line. Cloning and sequencing of the genomic regions surrounding the breakpoint junction of the deletion revealed that the homozygous deletion was caused by a simple interstitial deletion of a 220-kb segment. An AT-dinucleotide of contributing germline sequences was overlapped at the junction. Since there were one or two nucleotide overlaps of germline sequences at breakpoint junctions in all four cases of interstitial deletions analyzed to date, this may reflect a common mechanism underlying the occurrence of chromosomal interstitial deletion.

Keywords: chromosomal interstitial deletion; homozygous deletion; breakpoint junction; small cell lung carcinoma

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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