Journal Article

Genome Analysis of <i>Bacteroides</i> by Pulsed-field Gel Electrophoresis: Chromosome Sizes and Restriction Patterns

Syed Mohammed Shaheduzzaman, Shigeru Akimoto, Tomomi Kuwahara, Takemi Kinouchi and Yoshinari Ohnishi

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 4, issue 1, pages 19-25
Published in print January 1997 | ISSN: 1340-2838
Published online January 1997 | e-ISSN: 1756-1663 | DOI:

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The chromosomal DNAs of nine strains of seven Bacteroides species including B. fragilis, the type species of the genus Bacteroides, were digested with rare-cutting restriction enzymes I-Ceu I, Not I, and Asc I and analysed by pulsed-field gel electrophoresis. The genome sizes of B. fragilis, B. distasonis, B. eggerthii, B. ovatus, B. thetaiotaomicron, B. uniformis, and B. vulgatus were determined to be 5.3, 4.8, 4.4, 6.9, 4.8, 4.6, and 5.1 Mbp, respectively. B. distasonis and B. vulgatus, and also B. uniformis and B. eggerthii, showed simillar I-Ceu I restriction profiles. I-Ceu I cut B. uniformis and B. eggerthii genomes into four, B. ovatus into five, B. fragilis and B. thetaiotaomicron into six, and B. distasonis and B. vulgatus into seven fragments. On the basis of genome size, restriction profile, and I-Ceu I fragment number, a phylogenetic tree of the Bacteroides species was proposed. This was in overall agreement with the previous phylogenetic tree obtained by 16S rRNAdata, with the exceptions of B. distasonis and B. ovatus.

Keywords: Bacteroides species; PFGE; genome size; restriction pattern; phylogenetic relationship

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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