Journal Article

A 3-Mb Sequence-Ready Contig Map Encompassing the Multiple Disease Gene Cluster on Chromosome 11q13.1–q13.3

Eiko Kitamura, Fumie Hosoda, Michiyo Fukushima, Shuichi Asakawa, Nobuyoshi Shimizu, Takashi Imai, Eiichi Soeda and Misao Ohki

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 4, issue 4, pages 281-289
Published in print January 1997 | ISSN: 1340-2838
Published online January 1997 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/4.4.281

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Despite the presence of several human disease genes on chromosome 11q13, few of them have been molecularly cloned. Here, we report the construction of a contig map encompassing 11q13.1–q13.3 using bacteriophage P1 (P1), bacterial artificial chromosome (BAC), and P1-derived artificial chromosome (PAC). The contig map comprises 32 P1 clones, 27 BAC clones, 6 PAC clones, and 1 YAC clone and spans a 3-Mb region from D11S480 to D11S913. The map encompasses all the candidate loci of Bardet-Biedle syndrome type I (BBS1) and spinocerebellar ataxia type 5 (SCA5), one-third of the distal region for hereditary paraganglioma 2 (PGL2), and one-third of the central region for insulin-dependent diabetes mellitus 4 (IDDM4). In the process of map construction, 61 new sequence-tagged site (STS) markers were developed from the Not I linking clones and the termini of clone inserts. We have also mapped 30 ESTs on this map. This contig map will facilitate the isolation of polymorphic markers for a more re.ned analysis of the disease gene region and identi.cation of candidate genes by direct cDNA selection, as well as prediction of gene function from sequence information of these bacterial clones.

Keywords: chromosome 11; contig; SCA5; BBS1

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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