Journal Article

Prediction of the Coding Sequences of Unidentified Human Genes. VIII. 78 New cDNA Clones from Brain Which Code for Large Proteins <i>in vitro</i>

Ken-ichi Ishikawa, Takahiro Nagase, Daisuke Nakajima, Naohiko Seki, Miki Ohira, Nobuyuki Miyajima, Ayako Tanaka, Hirokazu Kotani, Nobuo Nomura and Osamu Ohara

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 4, issue 5, pages 307-313
Published in print January 1997 | ISSN: 1340-2838
Published online January 1997 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/4.5.307

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As a part of our project for accumulating sequence information of the coding regions of unidentified human genes, we herein report the sequence features of 78 new cDNA clones isolated from human brain cDNA libraries as those which may code for large proteins. The sequence data showed that the average size of the cDNA inserts andtheir open reading frames was 6.0 kb and 2.8 kb (925 amino acidresid ues), respectively, and these clones produced the corresponding sizes of protein products in an in vitro transcription/translation system. Homology search against the public databases indicated that the predicted coding sequences of 68 genes contained sequences similar to known genes, 69% of which (47 genes) were relatedto cell signaling/communication, nucleic acid management, and cell structure/motility. The expression profiles of these genes in 14 different tissues have been analyzed by the reverse transcription-coupled polymerase chain reaction method, and 8 genes were found to be predominantly expressed in the brain.

Keywords: large proteins; in vitro transcription/translation system; cDNA sequencing; expression profile; chromosomal location; brain

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Subjects: Genetics and Genomics

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