Journal Article

Sequence Analysis of the <i>groESL-cotA</i> Region of the <i>Bacillus subtilis</i> Genome, Containing the Restriction/Modification System Genes

Yasuhiro Kasahara, Sumiko Nakai, Naotake Ogasawara, Katsunori Yata and Yoshito Sadaie

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 4, issue 5, pages 335-339
Published in print January 1997 | ISSN: 1340-2838
Published online January 1997 | e-ISSN: 1756-1663 | DOI:

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We have determined a 35-kb sequence of the groESL-gutR-cotA (45°–52°) region of the Bacillus subtilis genome. In addition to the groESL, gutRB and cotA genes reported previously, we have newly identified 24 ORFs including gutA and fruC genes, encoding glucitol permease and fructokinase, respectively. The inherent restriction/modification system genes, hsdMR and hsdMM, were mapped between groESL and gutRB, and we have identified two open reading frames (ORFs) encoding 5-methylcytosine forming DNA methyl transferase and an operon probably encoding a restriction enzyme complex. The unusual genome structure of few ORFs and lower GC content around the restriction/modification genes strongly suggests that the region originated from a bacteriophage integrated during evolution.

Keywords: Bacillus subtilis; genome sequencing; glucitol operon; restriction/modification genes

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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