Journal Article

Isolation of cDNA for an NADP-malic Enzyme from <i>Aloe arborescens</i>

Hideo Honda, Hiroaki Shimada and Hiromori Akagi

in DNA Research

Published on behalf of Kazusa DNA Research Institute

Volume 4, issue 6, pages 397-400
Published in print January 1997 | ISSN: 1340-2838
Published online January 1997 | e-ISSN: 1756-1663 | DOI: http://dx.doi.org/10.1093/dnares/4.6.397

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NADP-malic enzyme catalyzes the reaction of decarboxylation from malate. In CAM plants, functions of this enzyme diverged to include both photosynthetic and non-photosynthetic roles. A full length cDNA for an NADP-malic enzyme was isolated from an ‘obligate’ CAM plant aloe (Aloe arborescens). The cDNA contains an ORF encoding 592 amino acid residues, whose sequence is highly homologous to the known plant NADP-malic enzymes. This gene is constitutively expressed in all organs in a low level. The amount of the transcript exhibited no diurnalv ariation, suggesting that this gene is not involved in photosynthetic functions.

Keywords: Aloe arborescence; CAM plant; NADP-malic enzyme; cDNA

Journal Article.  0 words. 

Subjects: Genetics and Genomics

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